SEAHORSE BIOSCIENCE XF96 EXTRACELLULAR FLUX ANALYZER w XF PREP STATION

Description

SEAHORSE BIOSCIENCE XF96 EXTRACELLULAR FLUX ANALYZER w XF PREP STATION
Product Specifics
Seahorse Bioscience XF96 Instrument
The Seahorse Bioscience XF96 instrument measures the rate of change of analytes
dissolved oxygen and pH in the media immediately surrounding living cells cultured in a
microplate. Changes in the extracellular media are caused by the consumption or
production of analytes by the cells. Therefore, a sensitive measurement of the media
flux can be used to determine rates of cellular metabolism with great precision and in a
totally non-invasive, label-free manner.
A unique feature of the XF technology is its ability to make accurate and repeatable
measurements in as little as five minutes. This is accomplished by isolating an
extremely small volume (less than 3 μl) of media above the cell monolayer. Cellular
metabolism causes rapid, easily measured changes to the “microenvironment” in this
small volume.
Typically, a measurement cycle is performed for 5-8 minutes. The media is gently
mixed and the analyte levels are then measured until the oxygen concentration drops
approximately 20-30% and media pH declines approximately 0.1-0.2 pH units. The
measurement is performed using 96 optical fluorescent biosensors embedded in a
sterile disposable cartridge that is placed into the Seahorse 96 well tissue culture
microplate.
Baseline metabolic rates are typically measured 3-4 times, and are reported in pmol/min
for OCR (and PPR) and in mpH/min for ECAR. Compound is then added to the media
and mixed, and then the post-treatment OCR and ECAR measurements are made and
repeated. As cells shift metabolic pathways, the relationship between OCR and ECAR
(PPR) changes.
Because XF measurements are non-destructive, cells can be profiled over a period of
minutes, hours or days.
The XF96 system include a bench top analyzer and touch screen controller, a plate
preparation station (PS) for exchanging media in the tissue culture plate, disposable
measurement / compound delivery cartridges, and microplates for cell culture,
calibration media and XF96 software.
Seahorse XF Prep Station
The Seahorse XF Prep Station is a compact, bench-top, fluid management system that prepares your culture plates for assay with the Seahorse XFe or XF Extracellular Flux Analyzers, saving valuable lab time, improving CV’s, and increasing the sensitivity of your ECAR measurements.
Contains a temperature controlled oven for incubating the cell culture plates in a 37°C/non-CO2 environment after the media exchange and a temperature controlled dry bath for warming and incubating the assay media
Fast, meticulous, and thorough washing of cells
Automated exchange of medium, ensuring consistent assay conditions
Integrated interface with the XF application software controls all wash steps sequenced within the XF assay protocol
User Manual
Unit's Specifics
The Seahorse Bioscience
XF96 System includes:
XF96 Analyzer
Make: Seahorse Bioscience
Model: XF96
Part No.: 100900-101
Mfg Date: 06/2010
Plate Preparation Station (PS)
Make: Seahorse Bioscience
Model: XF Prep Station
Part No.: 101200-100
Mfg Date: 05/2010
XF Controller
Tests and Observations
Seahorse XF96 Analyzer
Powers up
Communicates with XF Controller and initializes successfully (Current operation: Ready - as per the right side of the instrument and also on the XF Controller screen)
Temperature reaches the set point at 37ºC after warming up
Ran a few commands from Instrument Setup screen:
Move Tray Out: OK
Move Tray In: OK
Read Plate Barcode...: OK
Read Cartridge Barcode...: OK
Both instrument and plate tray temperatures are on and reaches their set points (+/-)
Seahorse XF Prep Station
Powers up
Communicates with XF Controller and initializes successfully (Status: Ready with green indicator on top right of the controller screen)
Both Incubator and Media temperatures reaches 37ºC set point successfully after warming up
Bottle empty indicators work well (Tested when bottles are empty and when they are filled with water)
Seahorse XF Controller
Touch display/ screen is tested to be responsive
Wireless keyboard and mouse are also tested to communicate and work properly
Communicates with both the XF96 Analyzer and Prep Station successfully
Comes with:
All necessary power and communication cables
Wireless Mouse/ Keyboard set
XF96 Calibrant Solution
Box of Seahorse XF96 Polystyrene Cell Culture Microplates (P/N 101085-004)
Box of XF96 Extracellular Flux Assay Kits
You will receive the actual/exact units as shown. Comes with all/only items/parts/components/accessories/cables shown
Description
This XF96 system is used and in well maintained, excellent physical condition.
The system powers up successfully with tests and observations recorded above and was pulled from a working research lab environment.
As we do not have the technical expertise to further test the system, we are selling it on an "AS IS BASIS". Inspection is available or if you'd like us to perform further checks/tests on the unit, please write to us with your set of instructions.
Shipping
We ship worldwide. Please email us for a shipping quote
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