PROTEINSIMPLE CELL BIOSCIENCES NANOPRO 100 CAPILLARY-BASED NANO-IMMUNOASSAY

Description

PROTEINSIMPLE CELL BIOSCIENCES NANOPRO 100 SIMPLE WESTERN CAPILLARY-BASED NANO-IMMUNOASSAY PLATFORM cIEF
Product Specifics
The ProteinSimple NanoPro™ systems enable detailed characterization of cell signaling processes in extremely small biological samples. Whereas traditional protein analysis techniques require thousands to millions of cells, NanoPro systems require as few as 25 cells per assay. Biological samples typically too small for traditional protein characterization techniques such as primary cells, fine needle tumor aspirates, FACS-sorted cells, microdissected tissue sections and isolated stem cell populations can be analyzed using straightforward protocols.
NanoPro systems are automated, capillary-based immunoassay platforms. As in western blot analysis, proteins from complex biological samples are separated, immobilized and probed with specific antibodies. However, NanoPro systems use capillary isoelectric focusing (cIEF) separation to resolve the various modification states of signaling proteins.
The capillary format provides rapid, high-resolution separations, with an average separation time of 40 minutes. After separation, proteins are chemically linked to the capillary wall. This linkage step is achieved through UV-light activation of a proprietary photoactive capture chemistry coated on the inner surface of each capillary. Proteins immobilized on the capillary wall are then washed and probed with primary and secondary antibodies.
Secondary antibodies are conjugated to horseradish peroxidase (HRP), which enables ultrasensitive chemiluminescence detection. After addition of Luminol and Peroxide detection reagents, chemiluminescence signal is collected through the capillary wall. Analysis is performed in Compass software and the resulting data are presented in electropherogram format along with a digital image of the separated proteins in the capillary.
Unit's Specifics
Make:
ProteinSimple/ Cell Biosciences
Model: Nanopro 100 (Simple Western System)
Type: Capillary-Based Nano-Immunoassay Platform
Note: You will receive the actual unit shown. Comes with all/ only components/ parts/ items/ accessories/ tubing shown
Description
This system is used, powers up as shown and is in overall good physical condition.
As we do not have the technical expertise to further test the unit, we are selling it on an "AS IS BASIS". Inspection is available, or if you'd like us to perform further tests/checks, please write to us with your set of instructions.
Shipping
We ship worldwide. Please email us for a shipping quote